Disclosed herein are devices and systems comprising a) a substrate comprising at least 100 microwells and a plurality of beads, wherein a plurality of the at least 100 microwells each contain a single bead, and wherein the ratio of the average diameter of the microwells to the diameter of the beads ranges from about 1.2 to about 1.8; b) a flow cell in fluid communication with the substrate; and c) at least one inlet port and at least one outlet port, wherein the at least one inlet port and at least one outlet port are capable of directing a flow of a fluid through the flow cell, thereby contacting the microwells with the fluid. In some embodiments, the at least one inlet port and at least one outlet port are in fluid communication with the flow cell via fluid channels having axes that form an angle relative to the plane of the substrate.
Fan, Christina (San Jose, CA, US) Fodor, Stephen P. (Palo Alto, CA, US) Fu, Glenn (Dublin, CA, US) Facer, Geoff (Redwood City, CA, US) Stern, David (Mountain View, CA, US) Lai, Janice (Mountain View, CA, US) Chaney, Ari (Palm Beach Gardens, FL, US) Spuhler, Phillip (Redwood City, CA, US) Li, Sixing (Redwood City, CA, US) Huang, Xiaohua Chen (Mountain View, CA, US) Metzler, Kimberly R. In some embodiments, the at least one inlet port and at least one outlet port are in fluid communication with the flow cell via fluid channels having axes that form an angle relative to the plane of the substrate, and wherein the angles for the fluid channels communicating with the inlet port and the outlet port are the same.
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(San Francisco, CA, US) Walczak, Elisabeth Marie (Menlo Park, CA, US) , and wherein a surface of the at least 100 microwells is coated with a surface coating to improve wettability; and a flow cell in fluid communication with the substrate. In some embodiments, the at least one inlet port and at least one outlet port are in fluid communication with the flow cell via fluid channels having axes that form an angle relative to the plane of the substrate, and wherein the angles for the fluid channels communicating with the inlet port and the outlet port are different.
In some embodiments, a surface of the at least 100 microwells comprises a plasma-treated surface.
Our quantitative PCR experiments also demonstrate that moa eggshell has approximately 125 times lower bacterial load than bone, making it a highly suitable substrate for high-throughput sequencing approaches.
Importantly, the preservation of DNA in Pleistocene eggshell from Australia and Holocene deposits from Madagascar indicates that eggshell is an excellent substrate for the long-term preservation of DNA in warmer climates. Holdaway, Eske Willerslev and Michael Bunce 1 Centre for Geo Genetics, University of Copenhagen , Universitetsparken 15, 2100 Copenhagen , Denmark 2 Ancient Biomolecules Centre, University of Oxford , Oxford OX1 3PS , UK 3 Western Australian Museum , Locked Bag 49, Welshpool DC, Western Australia 6986 , Australia 4 Ancient DNA Laboratory, School of Biological Sciences, Murdoch University , Perth, Western Australia 6150 , Australia 5 Department of Geological Sciences, University of Colorado , CO 80309-0450 , USA 6 Institute of Arctic and Alpine Research (INSTAAR) 7 School of Biological Sciences, University of Canterbury and Palaecol Research Ltd , Christchurch 8041 , New Zealand 8 The Centre for Microscopy, Characterization and Analysis, The University of Western Australia , Perth, Western Australia 6009 , Australia 9 Southern Pacific Archaeological Research, Department of Anthropology, University of Otago , Dunedin 9054 , New Zealand 10 Research Laboratory for Archaeology and the History of Art, University of Oxford , Oxford OX1 3QY , UK 11 Archaeology, University of Western Australia , Perth, Western Australia 6008 , Australia 12 Department of Archaeology, University of Sheffield , Sheffield S1 4ET , UK Articles on similar topics can be found in the following collections Receive free email alerts when new articles cite this article - sign up in the box at the top right-hand corner of the article or click here References Subject collections Email alerting service Fossil avian eggshell preserves ancient DNA Owing to exceptional biomolecule preservation, fossil avian eggshell has been used extensively in geochronology and palaeodietary studies.
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Nokia Bell Labs (formerly named AT&T Bell Laboratories, Bell Telephone Laboratories and Bell Labs) is an American research and scientific development company, owned by Finnish company Nokia.
Its headquarters are located in Murray Hill, New Jersey, in addition to other laboratories around the rest of the United States and in other countries.
The successful recovery of DNA from this substrate has implications in a number of scientific disciplines; most notably archaeology and palaeontology, where genotypes and/or DNA-based species identifications can add significantly to our understanding of diets, environments, past biodiversity and evolutionary processes. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a PDF plug-in installed and enabled in your browser. Holdaway 0 7 Eske Willerslev 0 1 Michael Bunce () 0 4 0 Charlotte L. Miller, Jean-Luc Schwenninger , Chris Jacomb, Richard Walter, Alexander Baynes, Joe Dortch, Michael Parker-Pearson, M. Here, we show, to our knowledge, for the first time that fossil eggshell is a previously unrecognized source of ancient DNA (a DNA). Fossil avian eggshell preserves a DNA formed, along with non-calcified membranes, as the egg passes through the oviduct. Because most of the organic matrix is intracrystalline, rather than intercrystalline as in most biominerals (Miller et al.